IN VIVO ADAPTATION OF CELL CULTURES OF VARIOUS GRAPE VARIETIES IN NON-STERILE CONDITIONS
Main Article Content
Abstract
The paper presents the results of the adaptation of various grape varieties in vivo grown in culture in vitro with further transplantation into a greenhouse for growth and development. It is shown that the growing conditions significantly affect the processes of growth and development. Grape sprouts grown by microclonal propagation were transplanted into a container – bags with perlite, followed by transfer to a greenhouse. Studies have shown that all 12 grape varieties have adapted well to greenhouse conditions. The height of the adapted plants reached 4 meters, single clusters were observed, with ripe berries.
Article Details
Section
Crop husbandry
References
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2. Batukaev A. A. Introduction to culture in vitro, reproduction and adaptation ex vitro of complex-resistant grape varieties / A. A. Batukaev, E. A. Sobralieva, M. Sh. Idrisova //Quarterly scientific and practical journal problems of the development of the agro-industrial complex of the region - №2 (38), - 2019. – Pp.25-30.
3. Batukaev A. A. Scientific substantiation of technologies for growing seedlings and ensuring the physiological needs of grapes in trace elements in the agroecological conditions of the Tersko-Kuma sands: monograph / Batukaev A. A., Magomadov A. S. – Grozny. - 167c.
4. Batukaev A. A. Improving the technology of growing grape seedlings and increasing the productivity of grape plantations / A. A. Batukaev, A. S. Magomadov, G. P. Malykh, M. S. Batukaev // Bulletin of the Chechen State University. - 2014. - No. 1. - pp. 223-227.
5. Batukaev A. A. Improving the technology of accelerated reproduction of grapes by in vitro method and the use of growth regulators in vitro and in vivo/ A. A. Batukaev Diss. at the sois. uch. art., Doctor of Agricultural Sciences Moscow -1999.408p
6. Batukaev M. S. The influence of growth regulators on the growth and development of explants of grapes and fruit crops in vitro / M. S. Batukaev, D. O. Palaeva, A. A. Batukaev //Quarterly scientific and practical journal problems of the development of the agro-industrial complex of the region - №2 (46), - 2021. – Pp.18-22.
7. Butenko R. G. Cellular engineering. Textbook for biological specialties of universities. / R. G. Butenko, M. V., Gusev, A. F. Kirkin, et al. - M.: Higher School, 1987.
8. Kalashnikova E. A. Obtaining cell cultures Clchoriumintybusl. In vitro as a source of inulin/ E. A. Kalashnikova, S. K. Temirbekova, Yu. V. Afanasyeva, et al.//Bulletin of Russian Agricultural Science – 2022. -No. 1. – pp. 37-41.
9. Kandelaki N. D. The influence of mineral fertilizers on the grape harvest and the quality of wine / N. D. Kandelaki, N. R. Kenchiatvili // Winemaking and viticulture. – M.: Food industry, 2011. – No. 6. – 33c.
10. Kudryashova A. G. Economic efficiency of the use of micro fertilizers on the Tersko-Kuma sands of the Chechen Republic / G. P. Malykh, A. S. Magomadov, T. A. Zubova, A. G. Kudryashova // Scientific heritage of Ya. I. Potapenko – the basis of modern science of grapes and wine: materials of the International scientific and Practical conference dedicated to the 110th anniversary of the birthday of Ya. I. Potapenko. – Novocherkassk: VNIIViV, 2014. – pp. 255-259.
11. Makarova A. G. The influence of macro- and micro-fertilizers on the productivity and quality of the table grape variety Augustine on sandy soils / A. G. Makarova //All–Russian Research Institute of Viticulture and Winemaking named after Ya. I. Potapenko - branch of the Federal State Budgetary Institution "Federal Rostov Agrarian Scientific Center" pp.80-85.
12. Pershina L. A. Methodological guide for the organization and conduct of work on the cultivation of isolated organs and tissues of plants/ L. A. Pershina. - Rotiprint of the Institute of Catalysis SB of the USSR Academy of Sciences, Novosibirsk, 1991. – p. 19.
13. Preservation of vegetatively propagated crops in in vitro and cryo collections. Methodical instructions /Edited by T. A. Gavrilenko 2nd ed. and dop. - St. Petersburg.: 2017. – 75c.